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A-kinase anchoring proteins, or AKAPs, target the cAMP-dependent protein kinase (PKA) and other protein kinases or phosphatases (e.g., PP1) to specific subcellular loci presumably for maximum enzyme efficacy. We are investigating the functional properties of AKAP95 and AKAP149, which are both implicated in the regulation of cell cycle progression. Of interest are also nuclear PP1 targeting subunits such as PNUTS, which may affect chromosome architecture at mitosis.
Video - Laser scanning confocal analysis of HeLa cell nuclei showing PNUTS (green) and sc35 (red).
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