Publications

 

 

2009

Sørensen, A.L., and Collas, P. 2009. Immunoprecipitation of methylated DNA. Methods Mol. Biol. 567, 249-62.


Lindeman, L.C., Vogt-Kielland, L.T., Aleström, P., and Collas, P. 2009. Fish'n ChIPs: chromatin immunoprecipitation in the zebrafish embryo. Methods Mol. Biol. 567, 75-86.


Dahl, J.A., and Collas, P. 2009. muChIP: chromatin immunoprecipitation for small cell numbers. Methods Mol. Biol. 567, 59-74.


Collas, P. 2009. The state-of-the-art of chromatin immunoprecipitation. Methods Mol. Biol. 567, 1-25.


Pewsey, E., Bruce, C., Georgiou, A.S., Jones, M., Baker, D., Ow, S.Y., Wright, P.C., Freberg, C.K., Collas, and Fazeli, A.P. 2009. Proteomics analysis of epithelial cells reprogrammed in cell-free extract. Mol. Cell Proteomics. 8, 1401-12.


Dahl, J.A., Reiner, A.H., and Collas, P. 2009. Fast genomic muChIP-chip from 1,000 cells. Genome Biol. 10, R13.


Rogne, M., Stokka, A.J., Tasken, K., Collas, P., and Küntziger, T. 2009. Mutually exclusive binding of PP1 and RNA to AKAP149 affects the mitochondrial network. Hum. Mol. Genet. 18, 978-87.


Collas, P. 2009. Epigenetic states in stem cells. Biochim. Biophys. Acta 1790, 900-5.


Noer, A., Lindeman, L.C., and Collas, P. 2009. Histone H3 modifications associated with differentiation and long-term culture of mesenchymal adipose stem cells. Stem Cell Dev. 18, 725-36.


2008


Dahl, J.A., Duggal, S., Coulston, N., Millar, D., Melki, J., Shahdafar, A., Brinchmann, J.E., and Collas, P. 2008. Genomic and epigenetic instability of human bone marrow mesenchymal stem cells cultured in autologous serum or fetal calf serum. Int. J. Dev. Biol. 52, 1033-42.

Ugland, H., Boquest, A.C., Naderi, S., Collas, P. and Blomhoff, H.K. 2008. cAMP-mediated induction of cyclin E sensitizes growth-arrested adipose stem cells to DNA damage-induced apoptosis. Mol. Biol. Cell. 19, 5082-92.

Shahdafar, A., Løken, S., Dahl, J.A., Tunheim, S.H., Eriksen, Collas, P., Reinholt, F.P., Engebretsen, L., and Brinchmann, J.E. 2008. Persistence of collagen type II in rapidly proliferating human articular chondrocytes in vitro. Tissue Eng. Part A 14, 1989-2007.

Dahl, J.A., and Collas, P. 2008. A rapid micro-chromatin immunoprecipitation assay. Nat. Protocols. 3, 1032-45.

Lund-Olesen, T., Dufva, M., Dahl, J.A., Collas, P., and Hansen, M.F. 2008. Sensitive on-chip quantitative real-time PCR performed on an adapatable and robust platform. Biomed. Microdevices. 10, 769-76.

Meza-Zepeda, L.A., Noer, A., Dahl, J.A., Micci, F., Myklebost, O., and Collas, P. 2008. High-resolution analysis of genetic stability of human adipose tissue stem cells cultured to senescence. J. Cell Mol. Med. 12, 553-63.

Håkelien, A.M., Delbarre, E., Gaustad, K.G., Buendia, B., and Collas, P. 2008. Expression of the myodystrophic R453W mutation of lamin A in C2C12 myoblasts causes promoter-specific and global epigenetic defects. Exp. Cell Res. 314, 1869-80.

Dahl, J.A., and Collas, P. 2008. MicroChIP – a rapid micro-chromatin immunoprecipitation assays for small cell numbers and biopsies. Nucl. Acids Res. 36, e15.

Collas, P., and Dahl, J.A. 2008. Chop it, ChIP it., check it: the status of chromatin immunoprecipitation. Front. Biosci. 13, 929-43.

Collas, P., Timoskainen, S., and Noer, A. 2008. Epigenetic basis for differentiation plasticity in stem cells. In Regulatory Networks in Stem Cells (V.K. Rajasekhar and Mohan C. Vemuri, Eds). Humana Press. In press.


2007

Dahl, J.A., and Collas, P. 2007. A quick and quantitative chromatin immunoprecipitation assay for small cell samples. Front. Biosci. 12, 4925-31.

 

Kvissel, A.K., Orstavik, S., Eikvar, S., Brede, G., Jahnsen, T., Collas, P., Akusjarvi, G., and Skalhegg, B.S. 2007. Involvement of the catalytic subunit of protein kinase A and of HA95 in pre-mRNA splicing. Exp. Cell Res. 313, 2795-809.

 

Freberg, C.T., Dahl, J.A., Timoskainen, S., and Collas, P. 2007. Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract. Mol. Biol. Cell. 18, 1543-53.

 

Collas, P., and Gammelsaeter, R. 2007. Novel approaches to epigenetic reprogramming of somatic cells. Cloning Stem Cells. 9, 26-32.

 

Dahl, J.A., and Collas, P. 2007. Q2ChIP, a quick and quantitative chromatin immunoprecipitation assay, unravels epigenetic dynamics of developmentally regulated genes in human carcinoma cells. Stem Cells. 25, 1037-46.

 

Noer, A., Boquest, A.C., and Collas, P. 2007. Dynamics of adipogenic promoter DNA methylation during clonal culture of human adipose stem cells to senescence. BMC. Cell Biol. 8, 18.

 

Boquest, A.C., Noer, A., Sorensen, A.L., Vekterud, K., and Collas, P. 2007. CpG methylation profiles of endothelial cell-specific gene promoter regions in adipose tissue stem cells suggest limited differentiation potential toward the endothelial cell lineage. Stem Cells. 25, 852-61.

 

Morrison, J.I., Loof, S., He, P., Alestrom, P., Collas, P., and Simon, A. 2007. Targeted gene delivery to differentiated skeletal muscle: a tool to study dedifferentiation. Dev. Dyn. 236, 481-8.

 

Collas, P. 2007. Dedifferentiation of cells: new approaches. Cytotherapy. 9, 236-44.

 

Collas, P., Noer, A., and Timoskainen, S. 2007. Programming the genome in embryonic and somatic stem cells. J. Cell. Mol. Med. 11, 602-20.


 

2006

Rogne, M., Landsverk, H.B., Van Eynde, A., Beullens, M., Bollen, M., Collas, P., and Küntziger, T. 2006. The KH-Tudor domain of A-Kinase anchoring protein 149 mediates RNA-dependent self-association. Biochemistry. 45, 14980-9.


Håkelien, A.M., Küntziger, T., Gaustad, K.G., Marstad, A., and Collas, P. 2006. In vitro reprogramming of nuclei and cells. Methods Mol. Biol. 348, 259-268.

Collas, P., and Taranger, C.T. 2006. Toward reprogramming cells to pluripotency. Ernst Schering Res. Found. Workshop. 60, 47-67.

Collas, P., Taranger, C.K., Boquest, A.C., Noer, A., and Dahl, J.A. 2006. On the way to reprogramming cells to pluripotency using cell-free extracts. Reprod. Biomed. Online. 12, 762-70.
                                                                                                                                                           Håkelien, A.M., Gaustad, K.G., and Collas, P. 2006. Modulation of cell fate using nuclear and cytoplasmic extracts. Methods Mol. Biol. 325, 99-114.

Boquest, A.C., Shahdadfar, A., Brinchmann, J.E., and Collas, P. 2006. Isolation of stromal stem cells from human adipose tissue. Methods Mol. Biol. 325, 35-46.

Noer, A.., Sørensen, A.L. , Boquest, A.C., and Collas, P. 2006. Stable CpG Hypomethylation of Adipogenic Promoters in Freshly Isolated, Cultured, and Differentiated Mesenchymal Stem Cells from Adipose Tissue. Mol. Biol. Cell. 17, 3543-56.

Küntziger, T., Rogne, M., Folstad, R.L., and Collas, P. 2006. Association of PP1 with its regulatory subunit AKAP149 is regulated by serine phosphorylation flanking the RVXF motif of AKAP149. Biochemistry. 45, 5868-77.

Collas P, Taranger CK, and Dahl JA. 2006. Epigenetic reprogramming of nuclei using nuclear and cytoplasmic extracts. Stem Cell Rev. 2, 309-317.

Boquest AC, Noer A, Sørensen AL, and Collas P. 2006. Epigenetic programming of mesenchymal stem cells from adipose tissue. Stem Cell Rev. 2, 319-329.


2005
Håkelien, A.M., Gaustad, K.G., Taranger, C.K., Skålhegg, B.S., Küntziger, T. and Collas, P. 2005. Long-term in vitro, cell-type-specific genome-wide reprogramming of gene expression.
Exp. Cell Res. 309, 32-47.

Qin, M., Tai, G., Collas, P., Polak, J.M. and Bishop, A.E. 2005. Cell extract-derived differentiation of embryonic stem cells. Stem Cells 23, 712-8.

Landsverk, H.B., Kirkhus, M., Bollen, M., Küntziger, T. and Collas, P. 2005. PNUTS enhances in vitro chromosome decondensation in a PP1-dependent  manner. Biochem. J. 390, 709-17.

Taranger, C.K., Noer, A., Sørensen, A.L., Håkelien, A.M., Boquest, A.B. and Collas, P. 2005. Induction of dedifferentiation, genome-wide transcriptional programming and epigenetic reprogramming by extracts of carcinoma and embryonic stem cells. Mol. Biol. Cell. 16, 5719-57.

Håkelien, A.M., Landsverk, H.B., Küntziger, T., Gaustad, K.G., and Collas, P. 2005. Reprogramming somatic nuclei and cells with cell extracts. In Cell Biology, A Laboratory Handbook (Celis, J. Ed), 207-212.

Boquest, A.C., Shahdadfar, A., Frønsdal K., Sigurjonsson, O., Tunheim, S., Collas, P. and Brinchmann, J.E. 2005. Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression following in vitro cell culture. Mol. Biol. Cell. 16, 1131-41.

2004
Sullivan, E.J., Kasinathan, R., Kasinathan, K., Robl, J.M., and Collas, P. 2004. Cloned calves from chromatin remodeled in vitro. Biol. Reprod. 70, 146-153.

Gaustad, K.G., Boquest, A.C., Anderson, B.E., Gerdes, A.M., and Collas, P. 2004. Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes. Biophys. Biochem. Res. Commun. 314, 420-427. 

Håkelien, A.M., Gaustad, K.G., and Collas, P. 2004. Transient alteration of cell fate using a nuclear and cytoplasmic extract of an insulinoma cell line. Biophys. Biochem. Res. Commun. 316, 834-841.

Collas, P., Küntziger, T, and Landsverk, H.B. 2004. Anchoring of protein kinase and phosphatase signaling units. In Topics Curr. Genet.: Protein Phosphatases (Ariño, J., and Alexander, D.R., Eds). Springer Verlag, Berlin, Heidelberg, New York. pp 145-166.

Collas, P., Martins, S., and Landsverk, H.B. 2004. Signaling to the nucleus via A-kinase anchoring proteins. In The Nuclear Envelope (Evans, D, Ed.). Bios Scientific Publishers, 247-265. 

Küntziger T., and Collas, P. 2004. Transdifferentiation. In Handbook of Adult and Fetal Stem Cells (Lanza, R. Ed). Academic Press, San Diego, USA. 2, 147-151.


2003                                                                                                    
Martins, S., Eikvar, S., Furukawa, K., and Collas, P. 2003. HA95 and LAP2beta mediate a novel chromatin-nuclear envelope interaction implicated in initiation of DNA replication. J. Cell Biol. 160, 177-188.

Steen, R.L., Beullens, M., Bollen, M., and Collas, P. 2003. AKAP149 is a novel PP1 specifier required to maintain nuclear envelope integrity in G1 phase. J. Cell Sci, 116, 2237-2246.

Eide, T., Tasken, K.A., Carlson, C.R., Williams, G., Tasken, K., and Collas, P. 2003. A-kinase anchoring protein AKAP95 interacts with MCM2, a component of the pre-replication complex. J. Biol. Chem. 278, 26750-26756.

Moreira, P., Robl, J.M., and Collas, P. 2003. Nuclear reprogramming defects in mouse nuclear transplant embryos. J. Cell Sci. 116, 3713-3720.

Martins, S., Marstad, A., and Collas, P. 2003. In vitro modulation of the interaction between HA95 and LAP2beta by cAMP signaling. Biochemistry 42, 10456-10461.

Collas, P., and Håkelien, A.M. 2003. Reprogramming somatic cells for therapeutic applications. E-biomed: The Journal of Regenerative Medicine 4, 7-13.

Collas, P., and Fissore, R.A. 2003. Activation of the mammalian egg. In Biology and Pathology of the Oocyte (eds. Trounson A. and Gosden, R.). Cambridge University Press, Cambridge, UK. pp 141-162.

Collas, P. 2003. Reprogramming cells for therapeutic applications: A novel avenue? European Tissue Repair Society Bulletin 10,112-114.

Collas, P., and Håkelien, A.M. 2003. Teaching cells new tricks. Trends Biotechnol. 21, 354-361.

Collas, P. 2003. Nuclear reprogramming in cell-free extracts. Phil. Trans. R. Soc. Lond. B. 358, 1389-1395.


2002
Landsverk, H.B., Håkelien, A.-M., Küntziger, T. Robl, J.M., Skålhegg, B.S., and Collas, P. 2002. Reprogrammed gene expression in a cell-free extract. EMBO Rep. 3, 384-389.

Håkelien, A.M., Landsverk, H.B., Robl, J.M., Skålhegg, B.S., and Collas, P. 2002. Reprogramming of fibroblast function in a cell-free extract: Nature Biotechnol. 20, 460-466.

Eide, T., Carlson, C.R., Tasken, K.A., Hirano, T., Tasken, K., and Collas, P. 2002. Distinct but overlapping domains of AKAP95 are implicated in chromosome condensation and condensin targeting. EMBO Rep. 3, 426-432.

Bomar, J., Moreira, P., Balise, J.J., and Collas, P. 2002. Differential regulation of maternal and paternal chromosome condensation in mitotic mouse zygotes. J. Cell Sci. 115, 2931-2940.

Burnside, A.S., and Collas, P. 2002. Induction of Oct-3/4 expression in somatic cells by gap junction-mediated signaling from blastomeres. Eur. J. Cell Biol. 81, 585-591.

Poccia, D.L., Barona, T., Collas, P., and Larijani. B. 2002. Nuclear envelope assembly in gametes and pronuclei. In Dynamics of Nuclear Envelope Assembly in Embryos and Somatic Cells. LANDES, Austin, TX, USA. pp 111-129.

Håkelien, A.M. and Collas, P. 2002. Novel approaches to transdifferentiation. Cloning and Stem Cells, 4, 379-387.


2001
Tasken, K.A., Collas, P.,
Kemmner, W.A., Witczak, O., Conti, M., and Tasken K. 2001. Phosphodiesterase 4D and protein kinase A type II constitute a signaling unit in the centrosomal area. J. Biol. Chem. 276, 21999-2002.

Fax, P., Carlson, C., Collas, P., Tasken, K., Esche H., and Brockmann D. 2001. Binding of PKA-RIIalpha to the adenovirus E1A oncoprotein correlates with its nuclear translocation and an increase in PKA-dependent promoter activity. Virology, 285, 30-41.

Landsverk, H.B., Carlson, C.R., Steen, R.S., Vossenbein, L., Herberg, F.W., Tasken, K., and Collas, P. 2001. Regulation of anchoring of the RIIalpha regulatory subunit of PKA to AKAP95 by phosphorylation of RIIalpha: implication on chromosome dynamics at mitosis. J. Cell Sci. 114, 3255-3264.

Carlson, C.R., Witczak, O., Vossenbein, L., Labbé, J.-C., Skålhegg, B.S., Keryer, G., Herberg, F.W., Collas, P., and Tasken, K. 2001. CDK1-mediated phosphorylation of the RIIalpha regulatory subunit of PKA works as a molecular switch that promotes dissociation of RIIalpha from centrosomes at mitosis. J. Cell Sci. 114. 3243-3254.

Steen, R.L., and Collas, P. 2001. Mistargeting of B-type lamins at the end of mitosis: Implications on cell survival and regulation of lamins A/C expression. J. Cell Biol. 153, 621-626.

Buendia, B., Courvalin, J.-C., and Collas, P. 2001. Nuclear envelope assembly and dynamics. Cell. and Molec. Life Sci. 58, 1781-1789.


2000
Ørstavik, S., Eide, T., Collas, P., Han, I.., Tasken, K., Kieff, E., Jahnsen, T., and Skålhegg, B.D. 2000. Identification, cloning and characterization of a novel nuclear protein, HA95, homologous to A-kinase anchoring protein 95. Biol. Cell. 92, 27-37.

Collas, P., Barona, T., and Poccia, D.L. 2000. Reorganization of sea urchin egg cytoplasmic membranes at fertilization. Eur. J. Cell Biol. 79, 10-16.

Reinton, N, Collas, P., Haugen, T., Skålhegg, B., Jahnsen, T., and Tasken, K. 2000. Cloning, characterization and subcellular localization of AKAP220 in the male germ line. Dev. Biol. 223, 194-204.

Steen, R.L., Cubizolles, F., Le Guellec, K., and Collas, P. 2000. AKAP95 recruits hCAP-D2/Eg7 for chromosome condensation in mitotic extract. J. Cell Biol. 149, 531-536.

Steen, R.S., Martins, S.M., Tasken, K., and Collas, P. 2000. Recruitment of PP1 at the nuclear envelope by A-kinase anchoring protein (AKAP)149 is a pre-requisite for nuclear lamina assembly. J. Cell Biol. 150, 1251-1261.

Martins, S., Eide, T., Steen, R.L., Jahnsen, T., Skållhegg, B.S., and Collas, P. 2000. HA95 is a major protein of the chromatin and nuclear matrix regulating nuclear breakdown at mitosis. J. Cell Sci. 113, 3703-3713.

Collas, P., and Poccia, D.L. 2000. Membrane fusion events during nuclear envelope assembly. Subcellular Biochem. 34, 273-302.

Collas, P. 2000. Formation of the sea urchin male pronucleus in cell-free extracts. Mol. Reprod. Devel. 56, 265-270.

Collas, P., and Courvalin, J.-C. 2000. Sorting nuclear membrane proteins at mitosis. Trends Cell Biol. 10, 5-8.